Protein microarray is one of the technology and it is a critical tool in biochemistry and molecular biology. The analytical microarray is the most powerful multiplexed detection technology. The functional microarray is one of the important tools for the high-throughput and large-scale system for biological studies. Some application of functional microarray is the detection of protein binding properties as protein-protein interaction, protein -DNA interaction, protein-RNA interaction and antigen-antibody interaction. There are numerous cellular processes in which protein kinases are involved. Recording and analyzing of immune responses.