PTMScan® Technology – Immunoaffinity enrichment for mass spectrometry-based proteomics
Mass spectrometry techniques can be used to profile post-translationally modified (PTM) peptides in a digested sample. However, PTM peptides are often present at very low abundance, which is a challenge when trying to study them using mass spectrometry techniques.
To address this challenge Cell Signaling Technology (CST™) has developed PTMScan® technology, a proprietary proteomic method that combines antibody enrichment of PTM-containing peptides with liquid chromatography mass spectrometry (LC-MS/MS). PTMScan technology allows identification and quantification of hundreds to thousands of even the lowest abundance peptides, and provides a more focused approach to peptide enrichment than other strategies (for example IMAC).
PTMScan technology can be used to:
- Determine novel protein sites that are phosphorylated, ubiquitinated, acylated, methylated, or protease cleaved.
- Identify and validate drug targets.
- Discover biomarkers.
- Explore the mechanism of action of drugs/chemical modulators.
- Elucidate off-target drug effects