Tuesday, 22 May 2018

Simplifying Proteomics

PTMScan® Technology – Immunoaffinity enrichment for mass spectrometry-based proteomics

Mass spectrometry techniques can be used to profile post-translationally modified (PTM) peptides in a digested sample. However, PTM peptides are often present at very low abundance, which is a challenge when trying to study them using mass spectrometry techniques.
To address this challenge Cell Signaling Technology (CST™) has developed PTMScan® technology, a proprietary proteomic method that combines antibody enrichment of PTM-containing peptides with liquid chromatography mass spectrometry (LC-MS/MS). PTMScan technology allows identification and quantification of hundreds to thousands of even the lowest abundance peptides, and provides a more focused approach to peptide enrichment than other strategies (for example IMAC).
PTMScan technology can be used to:
  • Determine novel protein sites that are phosphorylated, ubiquitinated, acylated, methylated, or protease cleaved.
  • Identify and validate drug targets.
  • Discover biomarkers.
  • Explore the mechanism of action of drugs/chemical modulators.
  • Elucidate off-target drug effects

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